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KMID : 0370220110550020168
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Yakhak Hoeji
2011 Volume.55 No. 2 p.168 ~ p.171
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Effects of Chrysosplenol C on Intracellular Ca²+ Transient in Isolated Rat Ventricular Myocytes
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Jung Suk-Han
Do Thi Thu Huong
Tran Van Sung
Nguyen Manh Cuong
Kim Young-Ho
Woo Sun-Hee
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Abstract
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Chrysosplenol C [5,6-dihydroxy-2-(4-hydroxy-3-methoxyphenyl)-3,7-dimethoxychromen-4-one] is a flavonoid found in Miliusa balansae and Pterocaulon sphacelatum. We have recently shown that chrysosplenol C has positive inotropic effect in isolated rat ventricular myocytes. In the present study, we explored a possible mechanism for the positive inotropic effect of chrysosplenol C by examining intracellular Ca²+ transients during action potentials. The intracellular Ca²+ transients were measured by confocal Ca²+ imaging in field-stimulated single rat ventricular myocytes. Chrysosplenol C (50 ㎛) significantly increased the magnitudes (△F/Fo) of Ca²+ transients (control, 1.08±0.05; chrysosplenol C, 1.25±0.03; n=8, P<0.01). Half decay time of the action potential-induced Ca²+ transient was not altered by chrysosplenol C (50 ㎛) (control, 154±6 ms; chrysosplenol C, 167±11 ms; n=21). The Ca²+ content in the sarcoplasmic reticulum (SR), measured as caffeine (10 mM)-induced Ca²+ transient, was significantly decreased by chrysosplenol C (50 ㎛). These results indicate that chrysosplenol C increases Ca²+ transients without altering Ca²+ removal kinetics in ventricular myocytes, providing a possible mechanism for its positive inotropic effect.
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KEYWORD
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chrysosplenol C, intercellular Ca²+ transient, ventricular myocytes, SR Ca²+ content, Ca²+ transient decay
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